Friday, April 25, 2008
Wednesday, April 23, 2008
Contact
Sandro Jube
University of Hawaii
Cancer Research Center
Department of Thoracic Oncology
651 Ilalo St., BSB 231B
Honolulu, HI 96813
Tel: (808) 440-4589
Fax: (808) 587-0790
sjube@crch.hawaii.edu
University of Hawaii
Cancer Research Center
Department of Thoracic Oncology
651 Ilalo St., BSB 231B
Honolulu, HI 96813
Tel: (808) 440-4589
Fax: (808) 587-0790
sjube@crch.hawaii.edu
Education
- Doctor of Philosophy (Ph.D.) in Molecular Biosciences and Bioengineering - University of Hawaii at Manoa (2009) (GPA 3.81)
- Master of Science (M.S.) Degree in Molecular Biosciences and Bioengineering - University of Hawaii at Manoa (2005) (GPA 3.67)
- Bachelor of Science (B.S.) Degree in Biology - Hawaii Pacific University (2001) (GPA 3.74 - Magna Cum Laude)
- Associate in Arts (A.A.) Degree in Liberal Art - Hawaii Tokai International College (1997) (GPA 4.0)
Experience
• Junior Researcher – Cancer Researcher Center of Hawaii, John A. Burns School of Medicine, University of Hawaii (September 2009 – Present). Conducting research on the mechanisms of mesothelioma carcinogenesis induced by asbestos exposure, with the purpose of developing new therapeutic and diagnostic tools against mesothelioma and other lung cancers.
• Adjunct Professor – Concepts of Genetics (BIOL 3050) Hawaii Pacific University (September 2009-December 2009). Upper-level genetics course focused on detailed view of the molecular processes that result in heredity, with emphasis on the effects of these processes on organism and evolution.
• Research Assistant - Department of Molecular Biosciences and Bioengineering at the University of Hawaii at Manoa, supervised by Dr. Dulal Borthakur (August 2002-August 2009).
- Genetic transformation of tobacco with Rhizobium genes for degradation of the toxic aromatic compound 3-hydroxy-4-pyridone.
- Development of an Agrobacterium-mediated transformation protocol for Leucaena leucocephala.
- Genetic engineering of Leucaena leucocephala for reduced mimosine content.
- Early stage development of a vaccine against Mycobacterium tuberculosis.
- Development of a new in vitro diagnostic test for Mycobacterium tuberculosis using peptide heteroconjugates as the stimulating molecules for T cells.
• Teaching Assistant - Biotechnology: Science and Ethical Issues (BIOL 304) University of Hawaii at Manoa (August 2005 – December 2005) - Introduction to the concepts, goals, ethical issues and consequences of biotechnology using real-life case studies of cloning, DNA fingerprinting, gene therapy and genetic engineering.
• Teaching Assistant - Introduction to General Biology Laboratory (BIOL 172L) University of Hawaii at Manoa (January 2003 – May 2003) - Anatomy, physiology, and systematics of plants and animals; behavior; ecosystems, populations, and communities.
• Manuscript Reviewing -
• Acted as the principal Reviewer for the journal Naturwissenschaften (Natural Sciences) (2008).
• Adjunct Professor – Concepts of Genetics (BIOL 3050) Hawaii Pacific University (September 2009-December 2009). Upper-level genetics course focused on detailed view of the molecular processes that result in heredity, with emphasis on the effects of these processes on organism and evolution.
• Research Assistant - Department of Molecular Biosciences and Bioengineering at the University of Hawaii at Manoa, supervised by Dr. Dulal Borthakur (August 2002-August 2009).
- Genetic transformation of tobacco with Rhizobium genes for degradation of the toxic aromatic compound 3-hydroxy-4-pyridone.
- Development of an Agrobacterium-mediated transformation protocol for Leucaena leucocephala.
- Genetic engineering of Leucaena leucocephala for reduced mimosine content.
- Early stage development of a vaccine against Mycobacterium tuberculosis.
- Development of a new in vitro diagnostic test for Mycobacterium tuberculosis using peptide heteroconjugates as the stimulating molecules for T cells.
• Teaching Assistant - Biotechnology: Science and Ethical Issues (BIOL 304) University of Hawaii at Manoa (August 2005 – December 2005) - Introduction to the concepts, goals, ethical issues and consequences of biotechnology using real-life case studies of cloning, DNA fingerprinting, gene therapy and genetic engineering.
• Teaching Assistant - Introduction to General Biology Laboratory (BIOL 172L) University of Hawaii at Manoa (January 2003 – May 2003) - Anatomy, physiology, and systematics of plants and animals; behavior; ecosystems, populations, and communities.
• Manuscript Reviewing -
• Acted as the principal Reviewer for the journal Naturwissenschaften (Natural Sciences) (2008).
Training/Certificates
- “Vertebrate Animal Care and Use/Laboratory Animal Safety Training” – 2006 (University of Hawaii at Manoa - Laboratory Animal Safety/LAS)
- “Protection of Human Subjects” – 2006 (University of Hawaii at Manoa)
- “Transportation of Dangerous Biological Goods” – 2006 (University of Hawaii at Manoa)
- “A Field Guide to GENBANK and NCBI Molecular Biology Resources” – 2005 (National Institutes of Health/National Center for Biotechnology Information)
- "Hazardous Waste Generator” – 2005 (University of Hawaii at Manoa)
- “Teaching Assistant Training and Development” – 2003 (University of Hawaii at Manoa)
- “Chemical Lab Safety” – 2002 (University of Hawaii at Manoa)
- “Biosafety Training” – 2002 (University of Hawaii at Manoa)
Awards & Honors
- First prize in PhD oral presentation at the 2008 Hawaii Chapter of American Society for Microbiology (ASM) Annual Meeting
- First prize in PhD poster presentation in the 2008 College of Tropical Agriculture and Human Resources Student Symposium (University of Hawaii at Manoa)
- National Science Foundation (NSF) Travel Grant – 20th North American Symbiotic Nitrogen Fixation Conference - 2007
- First prize in PhD oral presentation in the 2007 College of Tropical Agriculture and Human Resources Student Symposium (University of Hawaii at Manoa)
- First prize in MS oral presentation in the 2005 College of Tropical Agriculture and Human Resources Student Symposium (University of Hawaii at Manoa)
- Award of Merit for MS Student Oral Presentation in the 2004 CTAHR Symposium (University of Hawaii at Manoa)
- Gamma Sigma Delta Honor Society of Agriculture - 2005 (University of Hawaii at Manoa)
- Alpha Chi National College Honor Society - 1999 (Hawaii Pacific University)
- Beta Beta Beta National Biological Honor Society - Phi Kappa Chapter – 2000 (Hawaii Pacific University)
- Dean’s List – 1998, 1999, 2000 and 2001(Hawaii Pacific University)
- Presidential Academic Scholarship – 1998, 1999, 2000, 2001 (Hawaii Pacific University)
- The President’s Award for Academic Achievement - 1997 (Hawaii Tokai International College)
- Presidential Scholarship Award – 1997 (Hawaii Tokai International College)
Professional Organizations
- American Association for the Advancement of Science (AAAS)
- American Society of Plant Biology (ASPB)
- American Society of Microbiology (ASM)
- The Science, Technology, Engineering, and Mathematics (STEM) Education Coalition
Skills
• Experience in designing and optimizing molecular biology methods including: DNA, RNA and protein isolation from different plant and animal tissues; PCR; RT-PCR; Real-Time PCR (qPCR); molecular cloning; subtractive hybridization; Southern/Northern/Western blotting; ELISA; protein purification; mass spectrometry; liquid chromatography; cytotoxicity and proliferation assays; immunofluorescence; immunocytochemistry; immunohistochemistry; and others.
• Experience in animal and plant in vitro tissue culture.
• Institutional Animal Care and Use Committee (IACUC) certified small animal handler.
• Experience in the design, set-up and maintenance of experiments involving the growth of plants in controlled environmental chambers.
• Experience with the design and development of plant binary vectors for the generation of transgenic plants.
• Stable transformation of N. tabacum and L. leucocephala mediated by Agrobacterium tumefaciens.
• Computer skills: Microsoft Word; Excel; Power Point; Adobe Photoshop; Endnote; Vector NTI; GraphPad Prism and Integrative Bioinformatics Databases like NCBI.
• Experience in animal and plant in vitro tissue culture.
• Institutional Animal Care and Use Committee (IACUC) certified small animal handler.
• Experience in the design, set-up and maintenance of experiments involving the growth of plants in controlled environmental chambers.
• Experience with the design and development of plant binary vectors for the generation of transgenic plants.
• Stable transformation of N. tabacum and L. leucocephala mediated by Agrobacterium tumefaciens.
• Computer skills: Microsoft Word; Excel; Power Point; Adobe Photoshop; Endnote; Vector NTI; GraphPad Prism and Integrative Bioinformatics Databases like NCBI.
Publications
(Refereed Journals)
• Yang, H., Rivera, Z., Jube, S., Nasu, M., Bertino, P., Venkata, C.G., Franzoso, G., Lotze, M.T., Krausz, T., Pass, H.I., Bianchi, M.E., Carbone, M. (2010) Programmed necrosis induced by asbestos in human mesothelial cells causes HMGB1 release and resultant inflammation. Proceedings of the National Academy of Science of USA (PNAS Early Edition).
• Walton, C.B., Jube, S., Schorlemmer, A., Patek, P.Q., Zimmerman, D.H., Rosenthale, K.S., and Borthakur., D. (2010) Ex vivo stimulation assay for T-cell responses for tuberculosis using LEAPS-peptide heteroconjugates. Tuberculosis Research and Treatment (article submitted).
• Jube, S. and Borthakur, D. (2010) Transgenic Leucaena leucocephala expressing the Rhizobium gene pydA encoding a meta-cleavage dioxygenase shows reduced mimosine content. Plant Physiology and Biochemistry 48 (4)273-278.
• Jube, S. and Borthakur, D. (2009) Development of an Agrobacterium-mediated transformation protocol for the tree-legume Leucaena leucocephala using immature zygotic embryos. Plant Cell, Tissue and Organ Culture: Journal of Plant Biotechnology 96 (3)325-333.
• Jube, S., Awaya, J., and Borthakur, D. (2009) Expression of the Rhizobium pydA-pydB fusion gene in Nicotiana tabacum confers resistance to the toxic aromatic compound 3-hydroxy-4-pyridone. Biologia Plantarum 53 (2)355-359.
• Walton, C.B., Inos, A.B.H., Andres, O.A., Jube, S., de Couet, H.G., Douglas, J.T., Patek, P.Q., and Borthakur, D. (2008) Immunization with hybrid recombinant Mycobacterium tuberculosis H37Rv proteins increases the TH1 cytokine response in mice following a pulmonary instillation of irradiated mycobacteria. Vaccine 26 (34)4396-4402.
• Jube, S. and Borthakur, D. (2007) Expression of bacterial genes in transgenic tobacco: methods, applications and future prospects. Electronic Journal of Biotechnology 10 (3)452-467.
(Book Chapter)
• Jube, S. and Borthakur D. (2006) Recent advances in food biotechnology research. In: Hui YH, Nip W-K, Nollet LML, Paliyath G, Sahlstrøm S, and Simpson BK (eds) Food Biochemistry and Food Processing. pp 35-70, Blackwell Publishing, Oxford, U.K.
(Thesis and Dissertation)
• Jube, S. (2009) Genetic transformation of Leucaena leucocephala for reduction of mimosine content. University of Hawaii at Manoa, Doctor of Philosophy (Ph.D.) Dissertation, 119 pages.
• Jube, S. (2005) Genetic transformation of tobacco with Rhizobium genes for degradation of the toxic aromatic compound 3-hydroxy-4-pyridone. University of Hawaii at Manoa, Master of Sciences (M.Sc.) Thesis, 86 pages.
• Yang, H., Rivera, Z., Jube, S., Nasu, M., Bertino, P., Venkata, C.G., Franzoso, G., Lotze, M.T., Krausz, T., Pass, H.I., Bianchi, M.E., Carbone, M. (2010) Programmed necrosis induced by asbestos in human mesothelial cells causes HMGB1 release and resultant inflammation. Proceedings of the National Academy of Science of USA (PNAS Early Edition).
• Walton, C.B., Jube, S., Schorlemmer, A., Patek, P.Q., Zimmerman, D.H., Rosenthale, K.S., and Borthakur., D. (2010) Ex vivo stimulation assay for T-cell responses for tuberculosis using LEAPS-peptide heteroconjugates. Tuberculosis Research and Treatment (article submitted).
• Jube, S. and Borthakur, D. (2010) Transgenic Leucaena leucocephala expressing the Rhizobium gene pydA encoding a meta-cleavage dioxygenase shows reduced mimosine content. Plant Physiology and Biochemistry 48 (4)273-278.
• Jube, S. and Borthakur, D. (2009) Development of an Agrobacterium-mediated transformation protocol for the tree-legume Leucaena leucocephala using immature zygotic embryos. Plant Cell, Tissue and Organ Culture: Journal of Plant Biotechnology 96 (3)325-333.
• Jube, S., Awaya, J., and Borthakur, D. (2009) Expression of the Rhizobium pydA-pydB fusion gene in Nicotiana tabacum confers resistance to the toxic aromatic compound 3-hydroxy-4-pyridone. Biologia Plantarum 53 (2)355-359.
• Walton, C.B., Inos, A.B.H., Andres, O.A., Jube, S., de Couet, H.G., Douglas, J.T., Patek, P.Q., and Borthakur, D. (2008) Immunization with hybrid recombinant Mycobacterium tuberculosis H37Rv proteins increases the TH1 cytokine response in mice following a pulmonary instillation of irradiated mycobacteria. Vaccine 26 (34)4396-4402.
• Jube, S. and Borthakur, D. (2007) Expression of bacterial genes in transgenic tobacco: methods, applications and future prospects. Electronic Journal of Biotechnology 10 (3)452-467.
(Book Chapter)
• Jube, S. and Borthakur D. (2006) Recent advances in food biotechnology research. In: Hui YH, Nip W-K, Nollet LML, Paliyath G, Sahlstrøm S, and Simpson BK (eds) Food Biochemistry and Food Processing. pp 35-70, Blackwell Publishing, Oxford, U.K.
(Thesis and Dissertation)
• Jube, S. (2009) Genetic transformation of Leucaena leucocephala for reduction of mimosine content. University of Hawaii at Manoa, Doctor of Philosophy (Ph.D.) Dissertation, 119 pages.
• Jube, S. (2005) Genetic transformation of tobacco with Rhizobium genes for degradation of the toxic aromatic compound 3-hydroxy-4-pyridone. University of Hawaii at Manoa, Master of Sciences (M.Sc.) Thesis, 86 pages.
Communications to scientific meetings
• Borthakur, D., Jube, S., Pal, A., Negi, V., Awaya, J., and Fox, P. (2010) Expression of Rhizobium genes for mimosine degradation in transgenic Leucaena leucocephala. 21st North American Symbiotic Nitrogen Fixation Conference, Columbia, MO, USA.
• Pal, A., Jube, S. and Borthakur, D. (2010) Expression of Rhizobium genes in transgenic Leucaena leucocephala for reducing mimosine toxicity. International Meeting of the American Society of Plant Biologists (ASPB), Montreal, Quebec, Canada.
• Jube, S. and Borthakur, D. (2009) Genetic engineering of Leucaena leucocephala for reduction of mimosine content. International Meeting of the American Society of Plant Biologists (ASPB), Honolulu, HI, USA.
• Jube, S. and Borthakur, D. (2008) Development of an Agrobacterium-mediated transformation protocol for Leucaena leucocephala, a high-protein content leguminous tree. XX International Congress of Genetics (ICG), Berlin, Germany.
• Jube, S. and Borthakur, D. (2008) Expression of bacterial genes for the detoxification of Leucaena. Annual Meeting of the American Society for Microbiology (ASM), Honolulu, HI, USA.
• Jube, S. and Borthakur, D. (2008) Development of an Agrobacterium-mediated transformation protocol for the recalcitrant tree-legume Leucaena leucocephala, using immature zygotic embryos. 20th Annual CTAHR Research Symposium, Honolulu, HI, USA.
• Walton, C.B., Jube, S., Patek, P.Q. and Borthakur, D. (2008) Development of a new in vitro diagnostic test for Mycobacterium tuberculosis using peptide heteroconjugates as the stimulating molecules for T cells. SemBioSys Genetics Inc. Calgary, Alberta, Canada.
• Jube, S. and Borthakur, D. (2008) Development of an Agrobacterium-mediated transformation protocol for Leucaena leucocephala, a high-protein content leguminous tree. 13th International Biotechnology Symposium and Exhibition (IBS) Dalian, China.
• Jube, S., Awaya, J.D., and Borthakur, D. (2007) Genetic engineering of Leucaena leucocephala for reduced mimosine content using genes from Rhizobium sp. strain TAL1145. 20th International Symbiotic Nitrogen Fixation Conference, Milwaukee, WI, USA.
• Jube, S., Awaya, J.D. and Borthakur, D. (2007) Genetic engineering of Leucaena leucocephala for reduced mimosine content. 19th Annual CTAHR Research Symposium, Honolulu, HI, USA.
• Jube, S. and Borthakur, D. (2006) Development of an Agrobacterium-mediated transformation protocol for Leucaena Leucocephala, a high protein content leguminous tree. 18th Annual CTAHR Research Symposium, Honolulu, HI, USA.
• Borthakur, D. and Jube, S. (2005) Development of mimosine-free leucaena. School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
• Jube, S., Awaya, J.D. and Borthakur, D. (2005) Genetic transformation of tobacco with Rhizobium fusion genes for degradation of the toxic aromatic compound 3-hydroxy-4-pyridone. International Meeting of the American Society of Plant Biologists (ASPB), Seattle, WA, USA.
• Jube, S. and Borthakur, D. (2005) Genetic transformation of tobacco with Rhizobium genes for degradation of the toxic aromatic compound 3-hydroxy-4-pyridone. 17th Annual CTAHR Research Symposium, Honolulu, HI, USA.
• Jube, S. and Borthakur, D. (2004) Expression of bacterial HP-degrading genes in Nicotiana tabacum. 16th Annual CTAHR Research Symposium, Honolulu, HI, USA.
• Pal, A., Jube, S. and Borthakur, D. (2010) Expression of Rhizobium genes in transgenic Leucaena leucocephala for reducing mimosine toxicity. International Meeting of the American Society of Plant Biologists (ASPB), Montreal, Quebec, Canada.
• Jube, S. and Borthakur, D. (2009) Genetic engineering of Leucaena leucocephala for reduction of mimosine content. International Meeting of the American Society of Plant Biologists (ASPB), Honolulu, HI, USA.
• Jube, S. and Borthakur, D. (2008) Development of an Agrobacterium-mediated transformation protocol for Leucaena leucocephala, a high-protein content leguminous tree. XX International Congress of Genetics (ICG), Berlin, Germany.
• Jube, S. and Borthakur, D. (2008) Expression of bacterial genes for the detoxification of Leucaena. Annual Meeting of the American Society for Microbiology (ASM), Honolulu, HI, USA.
• Jube, S. and Borthakur, D. (2008) Development of an Agrobacterium-mediated transformation protocol for the recalcitrant tree-legume Leucaena leucocephala, using immature zygotic embryos. 20th Annual CTAHR Research Symposium, Honolulu, HI, USA.
• Walton, C.B., Jube, S., Patek, P.Q. and Borthakur, D. (2008) Development of a new in vitro diagnostic test for Mycobacterium tuberculosis using peptide heteroconjugates as the stimulating molecules for T cells. SemBioSys Genetics Inc. Calgary, Alberta, Canada.
• Jube, S. and Borthakur, D. (2008) Development of an Agrobacterium-mediated transformation protocol for Leucaena leucocephala, a high-protein content leguminous tree. 13th International Biotechnology Symposium and Exhibition (IBS) Dalian, China.
• Jube, S., Awaya, J.D., and Borthakur, D. (2007) Genetic engineering of Leucaena leucocephala for reduced mimosine content using genes from Rhizobium sp. strain TAL1145. 20th International Symbiotic Nitrogen Fixation Conference, Milwaukee, WI, USA.
• Jube, S., Awaya, J.D. and Borthakur, D. (2007) Genetic engineering of Leucaena leucocephala for reduced mimosine content. 19th Annual CTAHR Research Symposium, Honolulu, HI, USA.
• Jube, S. and Borthakur, D. (2006) Development of an Agrobacterium-mediated transformation protocol for Leucaena Leucocephala, a high protein content leguminous tree. 18th Annual CTAHR Research Symposium, Honolulu, HI, USA.
• Borthakur, D. and Jube, S. (2005) Development of mimosine-free leucaena. School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
• Jube, S., Awaya, J.D. and Borthakur, D. (2005) Genetic transformation of tobacco with Rhizobium fusion genes for degradation of the toxic aromatic compound 3-hydroxy-4-pyridone. International Meeting of the American Society of Plant Biologists (ASPB), Seattle, WA, USA.
• Jube, S. and Borthakur, D. (2005) Genetic transformation of tobacco with Rhizobium genes for degradation of the toxic aromatic compound 3-hydroxy-4-pyridone. 17th Annual CTAHR Research Symposium, Honolulu, HI, USA.
• Jube, S. and Borthakur, D. (2004) Expression of bacterial HP-degrading genes in Nicotiana tabacum. 16th Annual CTAHR Research Symposium, Honolulu, HI, USA.
References
- Dr. Dulal Borthakur
Professor, Graduate Chair
Dept. Molecular Biosciences and Bioengineering
University of Hawaii at Manoa
1955 East-West Rd. Room 218
Honolulu, HI, 96822 USA
Phone: (808) 956-8210
E-mail: dulal@hawaii.edu - Dr. Qing Li
Professor
Dept. Molecular Biosciences and Bioengineering
University of Hawaii at Manoa
1955 East-West Rd. Room 218
Honolulu, HI 96822 USA
Phone: (808) 956-2011
E-mail: qingl@hawaii.edu - Dr. Harry Ako
Professor, Department Chair
Dept. Molecular Biosciences and Bioengineering
University of Hawaii at Manoa
1955 East-West Rd. Room 218
Honolulu, HI 96822 USA
Phone: (808) 956-2012
E-mail: hako@hawaii.edu
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